Comparative UV Spectroscopic Method Analysis and Validation for Estimation of Rifaximin in Pharmaceutical Preparation

In the present research work absorption characteristics of rifaximin was studied and validated in different solvents visible region for its quantitative estimation bulk dosage forms by UV spectrophotometric method. The solution Rifaximin were prepared 0.1N HCL (pH 1.2), Phosphate buffer 6.8) & 7.4) showed maximum at wavelength 439 nm, 440 nm 433 respective solvents. drug obeyed Beer–Lambert’s law concentration range 2, 4, 6, 8, 10 µg/mL with coefficient correlation (r2) 0.998. method as per ICH guidelines. Statistical analysis proved that methods are repeatable specific said drug. These can be adopted routine or tablet form.
 Keywords: Rifaximin, HCL, phosphate buffer, spectrophotometer.